Products

Rabbit TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

ERB0126 Catalogue No.
TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Reactivity:
Rabbit
Range:
7.813-500pg/ml
Sensitivity:
4.688pg/ml
Product Name
Rabbit TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Catalogue No.
ERB0126
Alias
TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rabbit
UniProt ID
P04924
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10594
EDTA plasma(n=5)93-10397
heparin plasma(n=5)90-10296
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)88-98%86-104%86-105%
EDTA plasma(n=5)92-99%82-95%90-101%
heparin plasma(n=5)81-91%81-96%80-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only