- Product Name
- Mouse TNF-α (High sensitive Tumor Necrosis Factor Alpha) ELISA Kit
- Catalogue No.
- EM0183-HS
- Alias
- TNF-α ELISA Kit, High sensitive Tumor Necrosis Factor Alpha ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100pg/ml
- Sensitivity
- < 0.938pg/ml
- Species
- Mouse
- UniProt ID
- P06804
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 80-102 | 90 |
EDTA plasma(n=5) | 86-103 | 92 |
heparin plasma(n=5) | 88-100 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 81-103% | 81-97% | 90-105% |
EDTA plasma(n=5) | 90-101% | 90-101% | 94-98% |
heparin plasma(n=5) | 91-99% | 92-98% | 91-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only