Products

Mouse TNF-α (High sensitive Tumor Necrosis Factor Alpha) ELISA Kit

EM0183-HS Catalogue No.
TNF-α ELISA Kit, High sensitive Tumor Necrosis Factor Alpha ELISA Kit
Reactivity:
Mouse
Range:
1.563-100pg/ml
Sensitivity:
0.938pg/ml
Product Name
Mouse TNF-α (High sensitive Tumor Necrosis Factor Alpha) ELISA Kit
Catalogue No.
EM0183-HS
Alias
TNF-α ELISA Kit, High sensitive Tumor Necrosis Factor Alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100pg/ml
Sensitivity
< 0.938pg/ml
Species
Mouse
UniProt ID
P06804
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)80-10290
EDTA plasma(n=5)86-10392
heparin plasma(n=5)88-10095
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)81-103%81-97%90-105%
EDTA plasma(n=5)90-101%90-101%94-98%
heparin plasma(n=5)91-99%92-98%91-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only