- Product Name
- Human anti-TNF-α Antibody(Anti Tumor Necrosis Factor Alpha Antibody) ELISA Kit
- Catalogue No.
- EH4080
- Alias
- anti-TNF-α Antibody ELISA Kit, Anti Tumor Necrosis Factor Alpha Antibody ELISA Kit
- Detection Method
- HRP Indirect ELISA Sandwich
- Application
- anti-TNF-α Antibody ELISA Kit allows for the in vitro quantitative determination of anti-TNF-α Antibody concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Human
- UniProt ID
- P01375
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of anti-TNF-α Antibody and the recovery rates were calculated by comparing the measured value to the expected amount of anti-TNF-α Antibody in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-101 | 93 |
EDTA plasma(n=5) | 86-101 | 92 |
heparin plasma(n=5) | 89-105 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-TNF-α Antibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-103% | 89-105% | 86-101% |
EDTA plasma(n=5) | 86-98% | 88-93% | 86-98% |
heparin plasma(n=5) | 95-100% | 86-100% | 87-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only