- Product Name
- Human EDN1(Endothelin-1) ELISA Kit
- Catalogue No.
- EH0648
- Alias
- ET-1 ELISA Kit, EDN1 ELISA Kit, ET1 ELISA Kit, HDLCQ7 ELISA Kit, PPET1 ELISA Kit, Preproendothelin-1 ELISA Kit, endothelin 1 ELISA Kit, endothelin-1 ELISA Kit, preproendothelin-1 ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- EDN1 ELISA Kit allows for the in vitro quantitative determination of EDN1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.25-80pg/ml
- Sensitivity
- < 0.75pg/ml
- Species
- Human
- UniProt ID
- P05305
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of EDN1 and the recovery rates were calculated by comparing the measured value to the expected amount of EDN1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-102 | 93 |
EDTA plasma(n=5) | 90-104 | 96 |
heparin plasma(n=5) | 88-105 | 98 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EDN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-102% | 98-102% | 100-105% |
EDTA plasma(n=5) | 89-101% | 83-88% | 91-100% |
heparin plasma(n=5) | 83-98% | 81-95% | 80-91% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only