- Product Name
- Rat ET-1(Endothelin 1) ELISA Kit
- Catalogue No.
- ER0019
- Alias
- ET-1 ELISA Kit, EDN1 ELISA Kit, ET1 ELISA Kit, HDLCQ7 ELISA Kit, PPET1 ELISA Kit, Preproendothelin-1 ELISA Kit, endothelin 1 ELISA Kit, preproendothelin-1 ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- ET-1 ELISA Kit allows for the in vitro quantitative determination of ET-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.25-80pg/ml
- Sensitivity
- < 0.625pg/ml
- Species
- Rat
- UniProt ID
- P22388
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-99 | 95 |
EDTA plasma(n=5) | 85-103 | 92 |
heparin plasma(n=5) | 90-104 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-100% | 86-100% | 97-104% |
EDTA plasma(n=5) | 82-100% | 82-100% | 82-96% |
heparin plasma(n=5) | 90-98% | 80-99% | 84-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only