12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit 
EU3131
Catalogue No.
- 12-HETE ELISA Kit, 12-Hydroxyeicosatetraenoic Acid ELISA Kit
- Reactivity:
- Universal
- Range:
- 0.313-20ng/ml
- Sensitivity:
- 0.188ng/ml
- Product Name
- 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit
- Catalogue No.
- EU3131
- Alias
- 12-HETE ELISA Kit, 12-Hydroxyeicosatetraenoic Acid ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- 12-HETE ELISA Kit allows for the in vitro quantitative determination of 12-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Universal
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of 12-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 12-HETE in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 86-105 95 EDTA plasma(n=5) 87-98 94 heparin plasma(n=5) 88-101 96 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 12-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 86-104% 85-101% 94-105% EDTA plasma(n=5) 83-93% 82-100% 82-101% heparin plasma(n=5) 80-97% 84-99% 84-96% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only
