VB12(Vitamin B12) ELISA Kit
EU2620
Catalogue No.
- VB12 ELISA Kit, Vitamin B12 ELISA Kit
- Reactivity:
- Universal
- Range:
- 50-500pg/ml
- Sensitivity:
- 25pg/ml
- Product Name
- VB12(Vitamin B12) ELISA Kit
- Catalogue No.
- EU2620
- Alias
- VB12 ELISA Kit, Vitamin B12 ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- VB12 ELISA Kit allows for the in vitro quantitative determination of VB12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 50-500pg/ml
- Sensitivity
- < 25pg/ml
- Species
- Universal
- UniProt ID
- CAS:68-19-9
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of VB12 and the recovery rates were calculated by comparing the measured value to the expected amount of VB12 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-102 95 EDTA plasma(n=5) 81-102 94 heparin plasma(n=5) 82-101 94 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VB12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 80-96% 87-99% 94-103% EDTA plasma(n=5) 92-101% 90-96% 92-100% heparin plasma(n=5) 93-99% 91-99% 90-100% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only