- Product Name
- 5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit
- Catalogue No.
- EU2582
- Alias
- 5-HIAA ELISA Kit, 5-Hydroxyindoleacetic Acid ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- 5-HIAA ELISA Kit allows for the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Universal
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of 5-HIAA and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HIAA in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 88-100 | 95 |
EDTA plasma(n=5) | 85-98 | 93 |
heparin plasma(n=5) | 86-104 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HIAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 89-103% | 87-102% | 90-95% |
EDTA plasma(n=5) | 84-100% | 85-98% | 82-101% |
heparin plasma(n=5) | 81-98% | 83-93% | 87-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only