Rat Bckdk ([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit
ER6010
Catalogue No.
- Bckdk ELISA Kit, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase ELISA Kit, mitochondrial ELISA Kit, Branched-chain alpha-ketoacid dehydrogenase kinase ELISA Kit, BCKD-kinase ELISA Kit, BCKDHKIN ELISA Kit
- Reactivity:
- Rat
- Range:
- 78.125-5000pg/ml
- Sensitivity:
- 46.875pg/ml
- Product Name
- Rat Bckdk ([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit
- Catalogue No.
- ER6010
- Alias
- Bckdk ELISA Kit, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase ELISA Kit, mitochondrial ELISA Kit, Branched-chain alpha-ketoacid dehydrogenase kinase ELISA Kit, BCKD-kinase ELISA Kit, BCKDHKIN ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Bckdk ELISA Kit allows for the in vitro quantitative determination of Bckdk concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Rat
- UniProt ID
- Q00972
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Bckdk and the recovery rates were calculated by comparing the measured value to the expected amount of Bckdk in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 90-101 93 EDTA plasma(n=5) 87-103 96 heparin plasma(n=5) 89-105 95 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bckdk and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 92-103% 89-99% 90-104% EDTA plasma(n=5) 83-98% 84-99% 83-97% heparin plasma(n=5) 85-89% 80-100% 80-98% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only