- Product Name
- Rat VIM(Vimentin) ELISA Kit
- Catalogue No.
- ER1992
- Alias
- VIM ELISA Kit, Vimentin ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- VIM ELISA Kit allows for the in vitro quantitative determination of VIM concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Rat
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of VIM and the recovery rates were calculated by comparing the measured value to the expected amount of VIM in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 80-99 | 91 |
EDTA plasma(n=5) | 81-104 | 93 |
heparin plasma(n=5) | 85-99 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 80-102% | 90-102% | 87-103% |
EDTA plasma(n=5) | 91-100% | 96-100% | 95-98% |
heparin plasma(n=5) | 92-99% | 90-96% | 90-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only