Products

Rat NLRP3(Nod Like Receptor Pyrins-3) ELISA Kit

ER1965 Catalogue No.
NACHT, LRR and PYD domains-containing protein 3 ELISA Kit, Cold autoinflammatory syndrome 1 protein homolog ELISA Kit, Cryopyrin ELISA Kit, Mast cell maturation-associated-inducible protein 1 ELISA Kit, PYRIN-containing APAF1-like protein 1 ELISA Kit, Nalp3 ELISA Kit, Pypaf1 ELISA Kit, Nlrp3 ELISA Kit, NLRP3 ELISA Kit
Reactivity:
Rat
Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Product Name
Rat NLRP3(Nod Like Receptor Pyrins-3) ELISA Kit
Catalogue No.
ER1965
Alias
NACHT, LRR and PYD domains-containing protein 3 ELISA Kit, Cold autoinflammatory syndrome 1 protein homolog ELISA Kit, Cryopyrin ELISA Kit, Mast cell maturation-associated-inducible protein 1 ELISA Kit, PYRIN-containing APAF1-like protein 1 ELISA Kit, Nalp3 ELISA Kit, Pypaf1 ELISA Kit, Nlrp3 ELISA Kit, NLRP3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
 NLRP3 ELISA Kit allows for the in vitro quantitative determination of NLRP3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
D4A523
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of NLRP3 and the recovery rates were calculated by comparing the measured value to the expected amount of NLRP3 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-9894
EDTA plasma(n=5)87-10391
heparin plasma(n=5)85-10298
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NLRP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)92-105%85-98%86-101%
EDTA plasma(n=5)85-100%84-101%82-94%
heparin plasma(n=5)85-100%80-94%81-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only