- Product Name
- Rat NLRP3(Nod Like Receptor Pyrins-3) ELISA Kit
- Catalogue No.
- ER1965
- Alias
- NACHT, LRR and PYD domains-containing protein 3 ELISA Kit, Cold autoinflammatory syndrome 1 protein homolog ELISA Kit, Cryopyrin ELISA Kit, Mast cell maturation-associated-inducible protein 1 ELISA Kit, PYRIN-containing APAF1-like protein 1 ELISA Kit, Nalp3 ELISA Kit, Pypaf1 ELISA Kit, Nlrp3 ELISA Kit, NLRP3 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- NLRP3 ELISA Kit allows for the in vitro quantitative determination of NLRP3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Rat
- UniProt ID
- D4A523
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of NLRP3 and the recovery rates were calculated by comparing the measured value to the expected amount of NLRP3 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 88-98 | 94 |
EDTA plasma(n=5) | 87-103 | 91 |
heparin plasma(n=5) | 85-102 | 98 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NLRP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 92-105% | 85-98% | 86-101% |
EDTA plasma(n=5) | 85-100% | 84-101% | 82-94% |
heparin plasma(n=5) | 85-100% | 80-94% | 81-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only