- Product Name
- Rat Phex (Phosphate-regulating neutral endopeptidase PHEX) ELISA Kit
- Catalogue No.
- ER1948
- Alias
- PHEX protein ELISA Kit, PHEX ELISA Kit, Phex ELISA Kit, Phosphate-regulating neutral endopeptidase PHEX ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Phex ELISA Kit allows for the in vitro quantitative determination of Phex concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Rat
- UniProt ID
- O35812
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Phex and the recovery rates were calculated by comparing the measured value to the expected amount of Phex in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-101 | 93 |
EDTA plasma(n=5) | 87-100 | 93 |
heparin plasma(n=5) | 86-101 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phex and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 91-98% | 88-102% | 87-103% |
EDTA plasma(n=5) | 86-101% | 86-96% | 82-98% |
heparin plasma(n=5) | 80-100% | 88-98% | 81-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only