- Product Name
- Rat RF-IgG (Rheumatoid Factor IgG) ELISA Kit
- Catalogue No.
- ER1931
- Alias
- Rheumatoid Factor IgG ELISA Kit, RF-IgG ELISA Kit
- Detection Method
- Indirect ELISA
- Application
- RF-IgG ELISA Kit allows for the in vitro quantitative determination of RF-IgG concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 3.125-200IU/ml
- Sensitivity
- < 1.875IU/ml
- Species
- Rat
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of RF-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of RF-IgG in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-99 | 93 |
EDTA plasma(n=5) | 90-100 | 95 |
heparin plasma(n=5) | 89-102 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RF-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-104% | 86-105% | 90-105% |
EDTA plasma(n=5) | 82-101% | 83-101% | 84-98% |
heparin plasma(n=5) | 81-99% | 90-99% | 84-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only