- Product Name
- Rat β-EP(Beta-Endorphin) ELISA Kit
- Catalogue No.
- ER1441
- Alias
- β-EP ELISA Kit, Beta-Endorphin ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- β-EP ELISA Kit allows for the in vitro quantitative determination of β-EP concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Rat
- UniProt ID
- P01194
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of β-EP and the recovery rates were calculated by comparing the measured value to the expected amount of β-EP in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 91-101 | 97 |
EDTA plasma(n=5) | 87-101 | 92 |
heparin plasma(n=5) | 89-103 | 96 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-EP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-104% | 85-97% | 94-103% |
EDTA plasma(n=5) | 82-98% | 82-95% | 82-100% |
heparin plasma(n=5) | 81-99% | 83-99% | 81-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only