- Product Name
- Rat SPB(Pulmonary Surfatcant Associated Protein B) ELISA Kit
- Catalogue No.
- ER1351
- Alias
- SPB ELISA Kit, Pulmonary Surfactant Associated Protein B ELISA Kit, SFTPB ELISA Kit, SP-B ELISA Kit, SFTP3 ELISA Kit, Pulmonary surfactant-associated proteolipid SPL ELISA Kit, Phe ELISA Kit, 18 kDa pulmonary-surfactant protein ELISA Kit, 6 kDa protein ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- SPB ELISA Kit allows for the in vitro quantitative determination of SPB concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Rat
- UniProt ID
- P22355
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of SPB and the recovery rates were calculated by comparing the measured value to the expected amount of SPB in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-105 | 97 |
EDTA plasma(n=5) | 87-102 | 95 |
heparin plasma(n=5) | 87-98 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-103% | 86-102% | 85-105% |
EDTA plasma(n=5) | 84-98% | 82-97% | 83-96% |
heparin plasma(n=5) | 80-99% | 81-96% | 93-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only