- Product Name
- Rat PAF(Platelet Activating Factor) ELISA Kit
- Catalogue No.
- ER1226
- Alias
- PAF ELISA Kit, Platelet Activating Factor ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- PAF ELISA Kit allows for the in vitro quantitative determination of PAF concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Rat
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PAF and the recovery rates were calculated by comparing the measured value to the expected amount of PAF in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-102 | 92 |
EDTA plasma(n=5) | 86-103 | 96 |
heparin plasma(n=5) | 87-103 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 95-105% | 90-101% | 90-104% |
EDTA plasma(n=5) | 82-99% | 92-100% | 89-97% |
heparin plasma(n=5) | 81-94% | 82-96% | 83-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only