Products

Rat ON(Osteonectin) ELISA Kit

ER1211 Catalogue No.
ON ELISA Kit, BM-40 ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, osteonectin ELISA Kit, Osteonectin ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit, ELISA Kit
Reactivity:
Rat
Range:
31.25-2000pg/ml
Sensitivity:
18.75pg/ml
Product Name
Rat ON(Osteonectin) ELISA Kit
Catalogue No.
ER1211
Alias
ON ELISA Kit, BM-40 ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, osteonectin ELISA Kit, Osteonectin ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ON ELISA Kit allows for the in vitro quantitative determination of ON concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
P16975
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of ON and the recovery rates were calculated by comparing the measured value to the expected amount of ON in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10292
EDTA plasma(n=5)88-10497
heparin plasma(n=5)89-9593
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ON and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-105%85-105%95-102%
EDTA plasma(n=5)83-101%86-93%83-89%
heparin plasma(n=5)82-98%84-96%81-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only