- Product Name
- Rat ON(Osteonectin) ELISA Kit
- Catalogue No.
- ER1211
- Alias
- ON ELISA Kit, BM-40 ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, osteonectin ELISA Kit, Osteonectin ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- ON ELISA Kit allows for the in vitro quantitative determination of ON concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Rat
- UniProt ID
- P16975
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ON and the recovery rates were calculated by comparing the measured value to the expected amount of ON in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-102 | 92 |
EDTA plasma(n=5) | 88-104 | 97 |
heparin plasma(n=5) | 89-95 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ON and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-105% | 85-105% | 95-102% |
EDTA plasma(n=5) | 83-101% | 86-93% | 83-89% |
heparin plasma(n=5) | 82-98% | 84-96% | 81-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only