- Product Name
- Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit
- Catalogue No.
- ER1187
- Alias
- NF-κB p65 ELISA Kit, NFKB3 ELISA Kit, p65RelA ELISA Kit, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A ELISA Kit, nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 ELISA Kit, p65 ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65 ELISA Kit, p65 ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A ELISA Kit, avian ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A ELISA Kit, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3 ELISA Kit, p65 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- NF-κB p65 ELISA Kit allows for the in vitro quantitative determination of NF-κB p65 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Rat
- UniProt ID
- Q7TQN4
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of NF-κB p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-92 | 91 |
EDTA plasma(n=5) | 92-99 | 95 |
heparin plasma(n=5) | 92-103 | 99 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-104% | 89-102% | 88-100% |
EDTA plasma(n=5) | 82-94% | 89-100% | 91-100% |
heparin plasma(n=5) | 87-100% | 88-97% | 87-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only