- Product Name
- Rat NE(Neutrophil Elastase) ELISA Kit
- Catalogue No.
- ER1182
- Alias
- NE ELISA Kit, ELANE ELISA Kit, PMN elastase ELISA Kit, Medullasin ELISA Kit, Bone marrow serine protease ELISA Kit, Elastase-2 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- NE ELISA Kit allows for the in vitro quantitative determination of NE concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Rat
- UniProt ID
- P00774
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 91-104 | 98 |
EDTA plasma(n=5) | 86-103 | 95 |
heparin plasma(n=5) | 95-102 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 89-103% | 97-103% | 90-102% |
EDTA plasma(n=5) | 85-101% | 84-99% | 82-101% |
heparin plasma(n=5) | 86-100% | 81-99% | 83-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only