Products

Rat NE(Neutrophil Elastase) ELISA Kit

ER1182 Catalogue No.
NE ELISA Kit, ELANE ELISA Kit, PMN elastase ELISA Kit, Medullasin ELISA Kit, Bone marrow serine protease ELISA Kit, Elastase-2 ELISA Kit
Reactivity:
Rat
Range:
1.563-100ng/ml
Sensitivity:
0.938ng/ml
Product Name
Rat NE(Neutrophil Elastase) ELISA Kit
Catalogue No.
ER1182
Alias
NE ELISA Kit, ELANE ELISA Kit, PMN elastase ELISA Kit, Medullasin ELISA Kit, Bone marrow serine protease ELISA Kit, Elastase-2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NE ELISA Kit allows for the in vitro quantitative determination of NE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Rat
UniProt ID
P00774
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)91-10498
EDTA plasma(n=5)86-10395
heparin plasma(n=5)95-10297
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)89-103%97-103%90-102%
EDTA plasma(n=5)85-101%84-99%82-101%
heparin plasma(n=5)86-100%81-99%83-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only