- Product Name
- Rat HSP-70(Heat Shock Protein 70) ELISA Kit
- Catalogue No.
- ER1057
- Alias
- DAAP-21F2.7 ELISA Kit, FLJ54328 ELISA Kit, HSP70-1B ELISA Kit, HSP70-2 ELISA Kit, HSPA1A ELISA Kit, heat shock 70kD protein 1B ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- HSP-70 ELISA Kit allows for the in vitro quantitative determination of HSP-70 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Rat
- UniProt ID
- P0DMW0
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of HSP-70 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-70 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-105 | 95 |
EDTA plasma(n=5) | 91-100 | 96 |
heparin plasma(n=5) | 86-101 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-70 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-102% | 89-98% | 86-97% |
EDTA plasma(n=5) | 83-99% | 82-98% | 85-99% |
heparin plasma(n=5) | 81-99% | 86-98% | 92-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only