- Product Name
- Rat COL1(Collagen Type Ⅰ) ELISA Kit
- Catalogue No.
- ER0848
- Alias
- COL1 ELISA Kit, Alpha-1 type I collagen ELISA Kit, Collagen alpha-1 ELISA Kit, I chain ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- COL1 ELISA Kit allows for the in vitro quantitative determination of COL1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Rat
- UniProt ID
- P02454
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of COL1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-99 | 92 |
EDTA plasma(n=5) | 86-98 | 90 |
heparin plasma(n=5) | 88-100 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-103% | 86-101% | 86-104% |
EDTA plasma(n=5) | 85-98% | 84-95% | 82-99% |
heparin plasma(n=5) | 81-100% | 85-99% | 84-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only