Products

Rat C5(Complement Component 5) ELISA Kit

ER0785 Catalogue No.
C5 ELISA Kit
Reactivity:
Rat
Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Product Name
Rat C5(Complement Component 5) ELISA Kit
Catalogue No.
ER0785
Alias
C5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C5 ELISA Kit allows for the in vitro quantitative determination of C5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
P08650
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of C5 and the recovery rates were calculated by comparing the measured value to the expected amount of C5 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-10094
EDTA plasma(n=5)85-9990
heparin plasma(n=5)89-105100
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)92-100%85-100%92-103%
EDTA plasma(n=5)89-101%87-101%88-92%
heparin plasma(n=5)84-99%83-99%82-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only