- Product Name
- Rat AGRN(Agrin) ELISA Kit
- Catalogue No.
- ER0724
- Alias
- AGRN ELISA Kit, Agrin ELISA Kit, agrin proteoglycan ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- AGRN ELISA Kit allows for the in vitro quantitative determination of AGRN concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Rat
- UniProt ID
- P25304
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of AGRN and the recovery rates were calculated by comparing the measured value to the expected amount of AGRN in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-104 | 97 |
EDTA plasma(n=5) | 85-100 | 92 |
heparin plasma(n=5) | 90-105 | 97 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGRN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-104% | 93-101% | 90-104% |
EDTA plasma(n=5) | 83-94% | 84-101% | 85-97% |
heparin plasma(n=5) | 80-99% | 81-92% | 81-88% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only