- Product Name
- Rat Capn5(Calpain-5) ELISA Kit
- Catalogue No.
- ER0656
- Alias
- Capn5 ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- Capn5 ELISA Kit allows for the in vitro quantitative determination of Capn5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.781-50ng/ml
- Sensitivity
- < 0.469ng/ml
- Species
- Rat
- UniProt ID
- Q8R4C0
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Capn5 and the recovery rates were calculated by comparing the measured value to the expected amount of Capn5 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-99 | 92 |
EDTA plasma(n=5) | 85-102 | 96 |
heparin plasma(n=5) | 85-100 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Capn5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-101% | 86-98% | 90-103% |
EDTA plasma(n=5) | 86-94% | 85-97% | 89-97% |
heparin plasma(n=5) | 83-98% | 84-99% | 84-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only