- Product Name
- Rat Nt5c3l(Cytosolic 5′-nucleotidase III-like protein) ELISA Kit
- Catalogue No.
- ER0544
- Alias
- Nt5c3l ELISA Kit, cN-III-like protein ELISA Kit, Cytosolic 5'-nucleotidase III-like protein ELISA Kit
- Detection Method
- Competitive ELISA, Coated with Antigen
- Application
- Nt5c3l ELISA Kit allows for the in vitro quantitative determination of Nt5c3l concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Rat
- UniProt ID
- Q6AYP7
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Nt5c3l and the recovery rates were calculated by comparing the measured value to the expected amount of Nt5c3l in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-104 | 95 |
EDTA plasma(n=5) | 86-96 | 90 |
heparin plasma(n=5) | 86-99 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nt5c3l and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 91-104% | 86-99% | 88-105% |
EDTA plasma(n=5) | 84-96% | 83-99% | 83-98% |
heparin plasma(n=5) | 83-93% | 88-97% | 90-99% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only