Rat P4ha1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit
ER0436
Catalogue No.
- P4ha1 ELISA Kit, PHalphaI ELISA Kit
- Reactivity:
- Rat
- Range:
- 0.313-20ng/ml
- Sensitivity:
- 0.188ng/ml
- Product Name
- Rat P4ha1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit
- Catalogue No.
- ER0436
- Alias
- P4ha1 ELISA Kit, PHalphaI ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- P4ha1 ELISA Kit allows for the in vitro quantitative determination of P4ha1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Rat
- UniProt ID
- P54001
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of P4ha1 and the recovery rates were calculated by comparing the measured value to the expected amount of P4ha1 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 88-96 91 EDTA plasma(n=5) 90-102 97 heparin plasma(n=5) 85-103 96 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P4ha1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 87-103% 87-102% 85-104% EDTA plasma(n=5) 83-91% 85-100% 84-99% heparin plasma(n=5) 85-99% 82-98% 82-98% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only