Products

Rat Hcrt(Orexin) ELISA Kit

ER0368 Catalogue No.
Hcrt ELISA Kit, OX ELISA Kit, PPORX ELISA Kit, PPOX ELISA Kit, Orexin ELISA Kit, Hypocretin ELISA Kit, Hcrt ELISA Kit
Reactivity:
Rat
Range:
7.813-500pg/ml
Sensitivity:
4.688pg/ml
Product Name
Rat Hcrt(Orexin) ELISA Kit
Catalogue No.
ER0368
Alias
Hcrt ELISA Kit, OX ELISA Kit, PPORX ELISA Kit, PPOX ELISA Kit, Orexin ELISA Kit, Hypocretin ELISA Kit, Hcrt ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Hcrt ELISA Kit allows for the in vitro quantitative determination of Hcrt concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rat
UniProt ID
O55232
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of Hcrt and the recovery rates were calculated by comparing the measured value to the expected amount of Hcrt in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-10392
EDTA plasma(n=5)90-9693
heparin plasma(n=5)88-10294
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hcrt and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)98-104%85-102%85-92%
EDTA plasma(n=5)85-99%83-95%84-98%
heparin plasma(n=5)83-98%80-98%83-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only