- Product Name
- Rat Fgb(Fibrinogen beta chain) ELISA Kit
- Catalogue No.
- ER0214
- Alias
- Fgb ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Fgb ELISA Kit allows for the in vitro quantitative determination of Fgb concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.56-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Rat
- UniProt ID
- P14480
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Fgb and the recovery rates were calculated by comparing the measured value to the expected amount of Fgb in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 92-105 | 97 |
EDTA plasma(n=5) | 85-97 | 91 |
heparin plasma(n=5) | 91-105 | 98 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fgb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-101% | 85-105% | 90-102% |
EDTA plasma(n=5) | 83-96% | 83-99% | 83-95% |
heparin plasma(n=5) | 81-97% | 81-100% | 81-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only