Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

ER0191 Catalogue No.

Alias:: HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Reactivity:: Rat
Range:: 7.813-500pg/ml
Sensitivity:: 4.688pg/ml

Manual

Specifications
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FAQs

Product Name

Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Catalogue No.

ER0191

Alias

HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF-1α concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Species

Rat

UniProt ID

O35800

Standard curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HIF-1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF-1α in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-99	94
EDTA plasma(n=5)	87-103	96
heparin plasma(n=5)	88-99	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.