- Product Name
- Rat GSK3β(Glycogen synthase kinase-3 beta) ELISA Kit
- Catalogue No.
- ER0060
- Alias
- GSK3B ELISA Kit, GSK-3 beta ELISA Kit, Serine ELISA Kit, threonine-protein kinase GSK3B ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- GSK3β ELISA Kit allows for the in vitro quantitative determination of GSK3β concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Rat
- UniProt ID
- P18266
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of GSK3β and the recovery rates were calculated by comparing the measured value to the expected amount of GSK3β in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-104 | 99 |
EDTA plasma(n=5) | 86-103 | 97 |
heparin plasma(n=5) | 88-98 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GSK3β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-104% | 85-102% | 94-105% |
EDTA plasma(n=5) | 82-99% | 84-94% | 83-96% |
heparin plasma(n=5) | 83-97% | 84-93% | 82-94% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only