- Product Name
- Rat IFN-γ(Interferon γ) ELISA Kit
- Catalogue No.
- ER0012
- Alias
- IFN-γ ELISA Kit, IFNG ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, Type II Interferon ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- IFN-γ ELISA Kit allows for the in vitro quantitative determination of IFN-γ concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Rat
- UniProt ID
- P01581
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 96-101 | 98 |
EDTA plasma(n=5) | 85-101 | 93 |
heparin plasma(n=5) | 85-102 | 96 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 89-103% | 90-100% | 86-98% |
EDTA plasma(n=5) | 83-99% | 82-96% | 86-98% |
heparin plasma(n=5) | 82-98% | 83-99% | 83-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only