- Product Name
- Mouse Hes1 (Transcription factor HES-1) ELISA Kit
- Catalogue No.
- EM7177
- Alias
- Transcription factor HES-1 ELISA Kit, Hairy and enhancer of split 1 ELISA Kit, Hes1 ELISA Kit, Hes-1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Hes1 ELISA Kit allows for the in vitro quantitative determination of Hes1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Mouse
- UniProt ID
- P35428
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Hes1 and the recovery rates were calculated by comparing the measured value to the expected amount of Hes1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-100 | 94 |
EDTA plasma(n=5) | 86-103 | 99 |
heparin plasma(n=5) | 85-105 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hes1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-98% | 97-105% | 86-105% |
EDTA plasma(n=5) | 88-94% | 83-98% | 82-92% |
heparin plasma(n=5) | 80-91% | 82-95% | 82-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only