- Product Name
- Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2)ELISA Kit
- Catalogue No.
- EM2057
- Alias
- ERK1/2 ELISA Kit, Extracellular Signal Regulated Kinase 1/2 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- ERK1/2ELISA Kit allows for the in vitro quantitative determination of ERK1/2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.781-50ng/ml
- Sensitivity
- < 0.469ng/ml
- Species
- Mouse
- UniProt ID
- Q99519
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK1/2 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-98 | 90 |
EDTA plasma(n=5) | 93-103 | 99 |
heparin plasma(n=5) | 86-99 | 91 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-100% | 88-104% | 85-97% |
EDTA plasma(n=5) | 84-101% | 84-90% | 85-92% |
heparin plasma(n=5) | 83-97% | 80-95% | 83-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only