- Product Name
- Mouse Gpbar1 (G-protein coupled bile acid receptor 1) ELISA Kit
- Catalogue No.
- EM1917
- Alias
- G-protein coupled bile acid receptor 1 ELISA Kit, Membrane-type receptor for bile acids ELISA Kit, M-BAR ELISA Kit, Gpbar1 ELISA Kit, Tgr5 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Gpbar1 ELISA Kit allows for the in vitro quantitative determination of Gpbar1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Mouse
- UniProt ID
- Q80SS6
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Gpbar1 and the recovery rates were calculated by comparing the measured value to the expected amount of Gpbar1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-104 | 93 |
EDTA plasma(n=5) | 93-101 | 97 |
heparin plasma(n=5) | 89-93 | 91 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gpbar1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-103% | 86-104% | 88-105% |
EDTA plasma(n=5) | 84-101% | 82-100% | 85-100% |
heparin plasma(n=5) | 83-99% | 82-99% | 80-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only