- Product Name
- Mouse sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit
- Catalogue No.
- EM1364
- Alias
- sIL-6R ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- sIL-6R ELISA Kit allows for the in vitro quantitative determination of sIL-6R concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 12.5-800pg/ml
- Sensitivity
- < 7.5pg/ml
- Species
- Mouse
- UniProt ID
- P22272
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-105 | 97 |
EDTA plasma(n=5) | 88-102 | 92 |
heparin plasma(n=5) | 86-100 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-97% | 93-101% | 86-99% |
EDTA plasma(n=5) | 82-97% | 86-95% | 85-100% |
heparin plasma(n=5) | 81-100% | 84-95% | 80-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only