- Product Name
- Mouse SHH(Hedgehog Homolog, Sonic) ELISA Kit
- Catalogue No.
- EM1360
- Alias
- SHH ELISA Kit, HHG1 ELISA Kit, HHG-1 ELISA Kit, HLP3 ELISA Kit, HPE3 ELISA Kit, SMMCIsonic hedgehog homolog ELISA Kit, Drosophila ELISA Kit, sonic hedgehog ELISA Kit, sonic hedgehog homolog ELISA Kit, sonic hedgehog protein ELISA Kit, TPT ELISA Kit, TPTPS ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- SHH ELISA Kit allows for the in vitro quantitative determination of SHH concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Mouse
- UniProt ID
- Q62226
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of SHH and the recovery rates were calculated by comparing the measured value to the expected amount of SHH in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 89-105 | 98 |
EDTA plasma(n=5) | 91-102 | 99 |
heparin plasma(n=5) | 87-101 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 89-105% | 86-104% | 86-101% |
EDTA plasma(n=5) | 82-101% | 86-99% | 85-93% |
heparin plasma(n=5) | 81-97% | 80-100% | 82-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only