Products

Mouse PKB(Protein Kinase B) ELISA Kit

EM1294 Catalogue No.
PKB ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, Protein kinase B ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit
Reactivity:
Mouse
Range:
0.781-50ng/ml
Sensitivity:
0.469ng/ml
Product Name
Mouse PKB(Protein Kinase B) ELISA Kit
Catalogue No.
EM1294
Alias
PKB ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, Protein kinase B ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PKB ELISA Kit allows for the in vitro quantitative determination of PKB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Mouse
UniProt ID
P31750
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of PKB and the recovery rates were calculated by comparing the measured value to the expected amount of PKB in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-10598
EDTA plasma(n=5)86-10595
heparin plasma(n=5)89-10598
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)86-97%89-103%86-99%
EDTA plasma(n=5)84-96%85-99%83-101%
heparin plasma(n=5)85-100%84-96%88-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only