- Product Name
- Mouse PKB(Protein Kinase B) ELISA Kit
- Catalogue No.
- EM1294
- Alias
- PKB ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, Protein kinase B ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- PKB ELISA Kit allows for the in vitro quantitative determination of PKB concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.781-50ng/ml
- Sensitivity
- < 0.469ng/ml
- Species
- Mouse
- UniProt ID
- P31750
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of PKB and the recovery rates were calculated by comparing the measured value to the expected amount of PKB in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-105 | 98 |
EDTA plasma(n=5) | 86-105 | 95 |
heparin plasma(n=5) | 89-105 | 98 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 86-97% | 89-103% | 86-99% |
EDTA plasma(n=5) | 84-96% | 85-99% | 83-101% |
heparin plasma(n=5) | 85-100% | 84-96% | 88-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only