- Product Name
- Mouse C4(Complement Component 4) ELISA Kit
- Catalogue No.
- EM0883
- Alias
- C4 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- C4 ELISA Kit allows for the in vitro quantitative determination of C4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 1.563-100ng/ml
- Sensitivity
- < 0.938ng/ml
- Species
- Mouse
- UniProt ID
- P01029
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of C4 and the recovery rates were calculated by comparing the measured value to the expected amount of C4 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-97 | 94 |
EDTA plasma(n=5) | 85-105 | 96 |
heparin plasma(n=5) | 92-103 | 98 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 96-105% | 87-104% | 86-102% |
EDTA plasma(n=5) | 82-99% | 84-100% | 82-98% |
heparin plasma(n=5) | 84-98% | 86-99% | 80-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only