Products

Mouse AST(Aspartate Aminotransferase) ELISA Kit

EM0857 Catalogue No.
AST ELISA Kit
Reactivity:
Mouse
Range:
0.156-10ng/ml
Sensitivity:
0.094ng/ml
Product Name
Mouse AST(Aspartate Aminotransferase) ELISA Kit
Catalogue No.
EM0857
Alias
AST ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AST ELISA Kit allows for the in vitro quantitative determination of AST concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Q8BN82
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of AST and the recovery rates were calculated by comparing the measured value to the expected amount of AST in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10092
EDTA plasma(n=5)87-10094
heparin plasma(n=5)90-10497
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AST and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-102%87-91%95-105%
EDTA plasma(n=5)82-99%82-100%82-98%
heparin plasma(n=5)88-100%82-95%81-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only