- Product Name
- Mouse Amot(Angiomotin) ELISA Kit
- Catalogue No.
- EM0757
- Alias
- Amot ELISA Kit, AMOT ELISA Kit, KIAA1071 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Amot ELISA Kit allows for the in vitro quantitative determination of Amot concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Mouse
- UniProt ID
- Q8VHG2
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Amot and the recovery rates were calculated by comparing the measured value to the expected amount of Amot in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-99 | 94 |
EDTA plasma(n=5) | 88-101 | 94 |
heparin plasma(n=5) | 90-93 | 91 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amot and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-104% | 85-103% | 86-98% |
EDTA plasma(n=5) | 86-100% | 89-98% | 83-98% |
heparin plasma(n=5) | 81-99% | 80-99% | 81-95% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only