- Product Name
- Mouse Sema3a(Semaphorin-3A) ELISA Kit
- Catalogue No.
- EM0752
- Alias
- Sema3a ELISA Kit, SEMA3A ELISA Kit, Sema3A ELISA Kit, semaphorin 3A ELISA Kit, coll-1 ELISA Kit, collapsin 1 ELISA Kit, Hsema-I ELISA Kit, Hsema-III ELISA Kit, sema domain ELISA Kit, immunoglobulin domain ELISA Kit, Ig ELISA Kit, short basic domain ELISA Kit, secreted ELISA Kit, Sema III ELISA Kit, SEMA1 ELISA Kit, SEMAD ELISA Kit, SEMAIII ELISA Kit, SEMAL ELISA Kit, semaphorin D ELISA Kit, Semaphorin III ELISA Kit, semaphorin L ELISA Kit, semaphorin-3A ELISA Kit, SemD ELISA Kit, Semaphorin III ELISA Kit, Sema III ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Sema3a ELISA Kit allows for the in vitro quantitative determination of Sema3a concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Mouse
- UniProt ID
- O08665
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Sema3a and the recovery rates were calculated by comparing the measured value to the expected amount of Sema3a in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 92-102 | 98 |
EDTA plasma(n=5) | 89-105 | 96 |
heparin plasma(n=5) | 87-98 | 95 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sema3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-100% | 87-101% | 85-102% |
EDTA plasma(n=5) | 86-97% | 88-100% | 85-98% |
heparin plasma(n=5) | 84-99% | 84-95% | 81-88% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only