- Product Name
- Mouse Akr1b1(Aldose reductase) ELISA Kit
- Catalogue No.
- EM0742
- Alias
- Akr1b1 ELISA Kit, AR ELISA Kit, AKR1B1 ELISA Kit, Aldose reductase ELISA Kit, Aldo-keto reductase family 1 member B1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Akr1b1 ELISA Kit allows for the in vitro quantitative determination of Akr1b1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Mouse
- UniProt ID
- P45376
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of Akr1b1 and the recovery rates were calculated by comparing the measured value to the expected amount of Akr1b1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 92-105 | 97 |
EDTA plasma(n=5) | 87-105 | 95 |
heparin plasma(n=5) | 86-104 | 93 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Akr1b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-103% | 88-99% | 85-101% |
EDTA plasma(n=5) | 82-98% | 83-96% | 84-99% |
heparin plasma(n=5) | 85-100% | 83-89% | 88-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only