Products

Mouse Srd5a1(3-oxo-5-alpha-steroid 4-dehydrogenase 1) ELISA Kit

EM0651 Catalogue No.
Srd5a1 ELISA Kit, SR type 1 ELISA Kit, Steroid 5-alpha-reductase 1 ELISA Kit, S5AR 1 ELISA Kit, ELISA Kit
Reactivity:
Mouse
Range:
0.156-10ng/ml
Sensitivity:
0.094ng/ml
Product Name
Mouse Srd5a1(3-oxo-5-alpha-steroid 4-dehydrogenase 1) ELISA Kit
Catalogue No.
EM0651
Alias
Srd5a1 ELISA Kit, SR type 1 ELISA Kit, Steroid 5-alpha-reductase 1 ELISA Kit, S5AR 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Srd5a1 ELISA Kit allows for the in vitro quantitative determination of Srd5a1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Q68FF9
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of Srd5a1 and the recovery rates were calculated by comparing the measured value to the expected amount of Srd5a1 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-9791
EDTA plasma(n=5)87-10599
heparin plasma(n=5)87-10493
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Srd5a1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)89-105%89-101%89-102%
EDTA plasma(n=5)85-96%82-97%86-98%
heparin plasma(n=5)85-98%80-90%86-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only