- Product Name
- Mouse CD274(Programmed cell death 1 ligand 1) ELISA Kit
- Catalogue No.
- EM0399
- Alias
- CD274 ELISA Kit, PDCD1L1 ELISA Kit, PDCD1LG1 ELISA Kit, PD-L1 ELISA Kit, B7-H ELISA Kit, B7-H1 ELISA Kit, B7H1 ELISA Kit, CD274 antigen ELISA Kit, CD274 molecule ELISA Kit, B7 homolog 1 ELISA Kit, PDL1 ELISA Kit, PDCD1 ligand 1 ELISA Kit, programmed cell death 1 ligand 1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- CD274 ELISA Kit allows for the in vitro quantitative determination of CD274 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Mouse
- UniProt ID
- Q9EP73
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of CD274 and the recovery rates were calculated by comparing the measured value to the expected amount of CD274 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-97 | 93 |
EDTA plasma(n=5) | 88-103 | 98 |
heparin plasma(n=5) | 85-97 | 91 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD274 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-103% | 88-105% | 86-105% |
EDTA plasma(n=5) | 85-95% | 82-101% | 83-101% |
heparin plasma(n=5) | 83-96% | 86-100% | 80-93% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only