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Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

EM0183 Catalogue No.

Alias:: TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Reactivity:: Mouse
Range:: 3.906-250pg/ml
Sensitivity:: 2.344pg/ml

Manual

Specifications
Publications
FAQs

Product Name

Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Catalogue No.

EM0183

Alias

TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

TNF-α ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.906-250pg/ml

Sensitivity

< 2.344pg/ml

Species

Mouse

UniProt ID

Standard curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-105	96
EDTA plasma(n=5)	85-105	96
heparin plasma(n=5)	85-105	91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-105%	86-100%	93-105%
EDTA plasma(n=5)	84-99%	96-100%	82-100%
heparin plasma(n=5)	81-100%	82-98%	81-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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