- Product Name
- Mouse IL-5(Interleukin 5) ELISA Kit
- Catalogue No.
- EM0120
- Alias
- IL-5 ELISA Kit, IL5 ELISA Kit, EDF ELISA Kit, TRF ELISA Kit, E-CSF ELISA Kit, ESGF ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- IL-5 ELISA Kit allows for the in vitro quantitative determination of IL-5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Mouse
- UniProt ID
- P04401
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of IL-5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-5 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 90-100 | 94 |
EDTA plasma(n=5) | 88-105 | 95 |
heparin plasma(n=5) | 87-104 | 96 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 91-104% | 91-102% | 88-104% |
EDTA plasma(n=5) | 86-97% | 85-100% | 89-100% |
heparin plasma(n=5) | 83-99% | 80-100% | 80-97% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only