- Product Name
- Mouse GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
- Catalogue No.
- EM0089
- Alias
- GM-CSF ELISA Kit, Granulocyte Macrophage Colony Stimulating Factor ELISA Kit, GMCSF ELISA Kit, CSF2 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- GM-CSF ELISA Kit allows for the in vitro quantitative determination of GM-CSF concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Mouse
- UniProt ID
- P01587
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of GM-CSF and the recovery rates were calculated by comparing the measured value to the expected amount of GM-CSF in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 94-101 | 98 |
EDTA plasma(n=5) | 91-103 | 98 |
heparin plasma(n=5) | 86-104 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GM-CSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 88-99% | 85-98% | 89-104% |
EDTA plasma(n=5) | 84-98% | 86-101% | 84-100% |
heparin plasma(n=5) | 81-96% | 82-100% | 83-92% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only