Horse IL-1β(Interleukin 1 Beta)ELISA Kit
- Product Name
- Horse IL-1β(Interleukin 1 Beta)ELISA Kit
- Catalogue No.
- EHS0052
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- IL-1βELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Horse
- UniProt ID
- Q28386
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 88-103 99 EDTA plasma(n=5) 87-99 92 heparin plasma(n=5) 89-100 95 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 87-103% 87-104% 88-104% EDTA plasma(n=5) 84-93% 84-100% 89-97% heparin plasma(n=5) 85-99% 80-100% 84-92% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only