- Product Name
- Human MBD2(Methyl-CpG-binding domain protein 2) ELISA Kit
- Catalogue No.
- EH9990
- Alias
- Methyl-CpG-binding domain protein 2 ELISA Kit, MBD2 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- MBD2 ELISA Kit allows for the in vitro quantitative determination of MBD2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of MBD2 and the recovery rates were calculated by comparing the measured value to the expected amount of MBD2 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 91-101 | 97 |
EDTA plasma(n=5) | 88-101 | 95 |
heparin plasma(n=5) | 93-98 | 96 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MBD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 94-102% | 87-103% | 85-103% |
EDTA plasma(n=5) | 83-96% | 85-98% | 86-100% |
heparin plasma(n=5) | 85-98% | 80-100% | 81-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only