- Product Name
- Human E2F1(Transcription factor E2F1) ELISA Kit
- Catalogue No.
- EH8100
- Alias
- Transcription factor E2F1 ELISA Kit, E2F-1 ELISA Kit, PBR3 ELISA Kit, Retinoblastoma-associated protein 1 ELISA Kit, RBAP-1 ELISA Kit, Retinoblastoma-binding protein 3 ELISA Kit, RBBP-3 ELISA Kit, pRB-binding protein E2F-1 ELISA Kit, RBBP3 ELISA Kit, E2F1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- E2F1 ELISA Kit allows for the in vitro quantitative determination of E2F1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 15.625-1000pg/ml
- Sensitivity
- < 9.375pg/ml
- Species
- Human
- UniProt ID
- Q01094
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of E2F1 and the recovery rates were calculated by comparing the measured value to the expected amount of E2F1 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-100 | 92 |
EDTA plasma(n=5) | 91-99 | 95 |
heparin plasma(n=5) | 88-95 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2F1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 87-105% | 85-94% | 90-105% |
EDTA plasma(n=5) | 83-99% | 84-87% | 83-100% |
heparin plasma(n=5) | 80-100% | 81-100% | 83-98% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only